Small GTPase networks

Small GTPase networks

Complex biochemical networks control the activation of small GTPases in space and time.

The most important proteins organizing intracellular transport are small GTPases, molecular switches that can exist in an ON- and OFF-state regulating vesicle formation, transport and fusion. Importantly, the activities of these proteins are regulated in complex biochemical networks, which can give rise to emergent properties and collective behavior.

In this research project, we are using an in vitro reconstitution approach to understand the logic of the regulatory networks. This will help us to understand important questions related to vesicle transport, such as what determines directed transport of vesicles and how endosomes change their biochemical identity during maturation?


Albert, Lukasz, Gabriel, Christian, Zane


Christian Duellberg, Albert Auer, Nicola Canigova, Katrin Loibl, Martin Loose.
In vitro reconstitution reveals phosphoinositides as cargo-release factors and activators of the ARF6 GAP ADAP1
PNAS (2021), vol. 118 (1) e2010054118.

U. Bezeljak, H. Loya, B. Kaczmarek, T. E. Saunders and M. Loose. 
Stochastic activation and bistability in a Rab GTPase regulatory network.
PNAS (2020), vol. 117(12):6540-6549.

P. A. Nguyen*, A. C. Groen*, M. Loose, K. Ishihara, M. Wühr, C. M. Field, and T. J. Mitchison.
Spatial Organization of Cytokinesis Signaling Reconstituted in a Cell-Free System.
Science (2014) vol. 346, 244-247


This research project has received funding from the Human Frontiers Science Program (HFSP), Grant No. RGY0083/2016,